This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation.One of the proteins is overexpressed in E.coli as HA-tagged recombinant protein and cell-free extracts are immunoprecipitated in HA-affinity resin.Cell extracts are treated with nuclease to degrade DNA and RNA, which rules out nucleic

In most law enforcement cases, judgment debtors have behaviors of evading execution in China, which seriously affects the authority of legal judgments and the judiciary’s credibility.Characteristics analysis of judgment debtors plays a vital role in finding out the concealed property and improving efficiency in handling law enforcement cases